Multi-omics analysis of an in vitro photoaging model and protective effect of umbilical cord mesenchymal stem cell-conditioned medium.

ABSTRACT:

Background

Skin ageing caused by long-term ultraviolet (UV) irradiation is a complex biological process that involves multiple signalling pathways. Stem cell-conditioned media is believed to have anti-ageing effects on the skin. The purpose of this study was to explore the biological effects of UVB irradiation and anti-photoaging effects of human umbilical cord mesenchymal stem cell-conditioned medium (hUC-MSC-CM) on HaCaT cells using multi-omics analysis with a novel cellular photoaging model.

Methods

A cellular model of photoaging was constructed by irradiating serum-starved HaCaT cells with 20 mJ/cm² UVB. Transcriptomics and proteomics analyses were used to explore the biological effects of UVB irradiation on photoaged HaCaT cells. Changes in cell proliferation

SUBMITTER: Zou X 

PROVIDER: S-EPMC9438153 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature