SILAC-based quantitative proteomics to investigate the eicosanoid associated inflammatory response in activated macrophages.

ABSTRACT:

Background

Macrophages play a central role in inflammation by phagocytosing invading pathogens, apoptotic cells and debris, as well as mediating repair of tissues damaged by trauma. In order to do this, these dynamic cells generate a variety of inflammatory mediators including eicosanoids such as prostaglandins, leukotrienes and hydroxyeicosatraenoic acids (HETEs) that are formed through the cyclooxygenase, lipoxygenase and cytochrome P450 pathways. The ability to examine the effects of eicosanoid production at the protein level is therefore critical to understanding the mechanisms associated with macrophage activation.

Results

This study presents a stable isotope labelling with amino acids in cell culture (SILAC) -based proteomics strategy to quantify the changes in macroph

SUBMITTER: Brace N 

PROVIDER: S-EPMC9438320 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature