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The use of readily available laboratory tests for the identification of the emerging yeast Candida auris in Mexico.

ABSTRACT: Identification of the emerging multidrug-resistant yeast Candida auris is challenging. Here, we describe the role of the Mexico national reference laboratory Instituto de Diagnóstico y Referencia Epidemiológicos Dr. Manuel Martínez Báez (InDRE) and the Mexican national laboratory network in the identification of C. auris. Reference identification of six suspected isolates was done based on phenotypic and molecular laboratory methods, including growth in special media, evaluation of isolate micromorphology, and species-specific PCR and pan-fungal PCR and sequencing. The four C. auris isolates identified were able to grow on modified Sabouraud agar with 10% NaCl incubated at 42 °C. With one exception, isolates of C. auris were spherical to ovoid yeast-like cells and blastoconidia, with no hyphae or pseudohyphae on cornmeal agar. C. auris isolates were resistant to fluconazole. Species-specific and pan-fungal PCR confirmed isolates as C. auris. Sequence analysis revealed the presence of two different C. auris clades in Mexico, clade I (South Asia) and clade IV (South America).

SUBMITTER: Gonzalez-Duran E

PROVIDER: S-EPMC9438372 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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The use of readily available laboratory tests for the identification of the emerging yeast Candida auris in Mexico.

González-Durán Elizabeth E Contreras-Pérez Cudberto U CU Caceres Diego H DH Ríos-Rosas Claudia C Piñón-Ortega Javier de J JJ Téllez-Saucedo Ma Dolores MD Marín-Suro Edgar S ES Wong-Arámbula Claudia E CE Moreno-Escobar E Alejandra EA Ramírez-González José Ernesto JE Ramírez-Barrios J Gabriela JG Montes-Colima Norma A NA Lockhart Shawn R SR Martínez-Montiel Nancy N Martínez-Contreras Rebeca D RD García-Ruíz Pablo P Salazar-Sánchez Ma Isabel MI Hernández-Rivas Lucia L López-Martínez Irma I

Archives of microbiology 20220902 9

Identification of the emerging multidrug-resistant yeast Candida auris is challenging. Here, we describe the role of the Mexico national reference laboratory Instituto de Diagnóstico y Referencia Epidemiológicos Dr. Manuel Martínez Báez (InDRE) and the Mexican national laboratory network in the identification of C. auris. Reference identification of six suspected isolates was done based on phenotypic and molecular laboratory methods, including growth in special media, evaluation of isolate micro ...[more]

PMID: 36053373

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Project description:Candida auris is a recently described pathogenic fungus that is causing invasive outbreaks on all continents. The fungus is of high concern given the numbers of multidrug-resistant strains that have been isolated in distinct sites across the globe. The fact that its diagnosis is still problematic suggests that the spreading of the pathogen remains underestimated. Notably, the molecular mechanisms of virulence and antifungal resistance employed by this new species are largely unknown. In the present work, we compared two clinical isolates of C. auris with distinct drug susceptibility profiles and a Candida albicans reference strain using a multi-omics approach. Our results show that, despite the distinct drug resistance profile, both C. auris isolates appear to be very similar, albeit with a few notable differences. However, compared to C. albicans both C. auris isolates have major differences regarding their carbon utilization and downstream lipid and protein content, suggesting a multifactorial mechanism of drug resistance. The molecular profile displayed by C. auris helps to explain the antifungal resistance and virulence phenotypes of this new emerging pathogen.IMPORTANCECandida auris was first described in Japan in 2009 and has now been the cause of significant outbreaks across the globe. The high number of isolates that are resistant to one or more antifungals, as well as the high mortality rates from patients with bloodstream infections, has attracted the attention of the medical mycology, infectious disease, and public health communities to this pathogenic fungus. In the current work, we performed a broad multi-omics approach on two clinical isolates isolated in New York, the most affected area in the United States and found that the omic profile of C. auris differs significantly from C. albicans In addition to our insights into C. auris carbon utilization and lipid and protein content, we believe that the availability of these data will enhance our ability to combat this rapidly emerging pathogenic yeast.

Dataset Information

The use of readily available laboratory tests for the identification of the emerging yeast Candida auris in Mexico.

Publications

The use of readily available laboratory tests for the identification of the emerging yeast Candida auris in Mexico.

Similar Datasets

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