Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein-level changes.

ABSTRACT: Meiotic cells introduce a numerous programmed DNA breaks into their genome to stimulate meiotic recombination and ensure controlled chromosome inheritance and fertility. A checkpoint network involving key kinases and phosphatases coordinates the repair of these DNA breaks, but the precise phosphorylation targets remain poorly understood. It is also unknown whether meiotic DNA breaks change gene expression akin to the canonical DNA-damage response. To address these questions, we analyzed the meiotic DNA break response in Saccharomyces cerevisiae using multiple systems-level approaches. We identified 332 DNA break-dependent phosphorylation sites, vastly expanding the number of known events during meiotic prophase. Less than half of these events occurred in recognition motifs for the k

SUBMITTER: Kar FM 

PROVIDER: S-EPMC9438802 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature