Native plasmids of Fusobacterium nucleatum: characterization and use in development of genetic systems.

ABSTRACT: Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.

SUBMITTER: Haake SK 

PROVIDER: S-EPMC94399 | biostudies-literature | 2000 Feb

REPOSITORIES: biostudies-literature