Mitochondrial Ca²⁺ uptake by the MCU facilitates pyramidal neuron excitability and metabolism during action potential firing.

ABSTRACT: Neuronal activation is fundamental to information processing by the brain and requires mitochondrial energy metabolism. Mitochondrial Ca²⁺ uptake by the mitochondrial Ca²⁺ uniporter (MCU) has long been implicated in the control of energy metabolism and intracellular Ca²⁺ signalling, but its importance to neuronal function in the brain remains unclear. Here, we used in situ electrophysiology and two-photon imaging of mitochondrial Ca²⁺, cytosolic Ca²⁺, and NAD(P)H to test the relevance of MCU activation to pyramidal neuron Ca²⁺ signalling and energy metabolism during action potential firing. We demonstrate that mitochondrial Ca²⁺ uptake by the MCU is tuned to enhanced firing rate and the strength of this relationsh

SUBMITTER: Groten CJ 

PROVIDER: S-EPMC9440007 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature