CDC42 governs normal oviduct multiciliogenesis through activating AKT to ensure timely embryo transport.

ABSTRACT: Ciliated and secretory cells are two major cell types that comprise the oviduct epithelia. Accumulating evidences support a role of oviductal multiciliated epithelia for embryo transport, however the mechanisms underlying this specialized cell type differentiation remain elusive. Here, we report that CDC42 depletion in oviduct epithelia hampers the morphogenesis of multiciliated cell, and results in embryo retention, leading to early pregnancy failure. Utilizing the oviduct organoid model, we further observed that CDC42 guides secretory cells transition into multiciliated cells independent of its GTPase activity and the well-known Notch pathway. Further exploration uncovered the AKT as a novel indispensable regulator for multiciliated cells differentiation, whose activity was maintained by

SUBMITTER: Jiang R 

PROVIDER: S-EPMC9440026 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature