Comparative maternal protein profiling of mouse biparental and uniparental embryos.

ABSTRACT:

Background

Maternal proteins have important roles during early embryonic development. However, our understanding of maternal proteins is still very limited. The integrated analysis of mouse uniparental (parthenogenetic) and biparental (fertilized) embryos at the protein level creates a protein expression landscape that can be used to explore preimplantation mouse development.

Results

Using label-free quantitative mass spectrometry (MS) analysis, we report on the maternal proteome of mouse parthenogenetic embryos at pronucleus, 2-cell, 4-cell, 8-cell, morula, and blastocyst stages and highlight dynamic changes in protein expression. In addition, comparison of proteomic profiles of parthenogenotes and fertilized embryos highlights the different fates of maternal proteins. Enri

SUBMITTER: Chen F 

PROVIDER: S-EPMC9440387 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature