Itaconic acid production is regulated by LaeA in Aspergillus pseudoterreus.

ABSTRACT: The global regulator LaeA controls secondary metabolism in diverse Aspergillus species. Here we explored its role in regulation of itaconic acid production in Aspergillus pseudoterreus. To understand its role in regulating metabolism, we deleted and overexpressed laeA, and assessed the transcriptome, proteome, and secreted metabolome prior to and during initiation of phosphate limitation induced itaconic acid production. We found that secondary metabolite clusters, including the itaconic acid biosynthetic gene cluster, are regulated by laeA and that laeA is required for high yield production of itaconic acid. Overexpression of LaeA improves itaconic acid yield at the expense of biomass by increasing the expression of key biosynthetic pathway enzymes and attenuat

SUBMITTER: Pomraning KR 

PROVIDER: S-EPMC9440423 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature