Rejuvenated endothelial progenitor cells through overexpression of cellular prion protein effectively salvaged the critical limb ischemia in rats with preexisting chronic kidney disease.

ABSTRACT:

Background

This study tested the hypothesis that overexpression of cellular prion protein in endothelial progenitor cells (PrPc^OE-EPCs), defined as "rejuvenated EPCs," was superior to EPCs for salvaging the critical limb ischemia (CLI) induced after 28-day chronic kidney disease (CKD) induction in rat.

Methods and results

Cell viability and flow cytometric analyses of early/late apoptosis/total-intracellular ROS/cell cycle (sub-G1, G2/M phase) were significantly higher in EPCs + H₂O₂ than in EPCs that were significantly reversed in PrPc^OE-EPCs + H₂O₂ (all p < 0.001). The protein expressions of inflammation (IL-1ß/IL-6/MMP-9/p-NF-κB) were significantly increased in EPC + TNF-α than in EPCs that were significant

SUBMITTER: Yeh JP 

PROVIDER: S-EPMC9440498 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature