Early transcriptional responses of bronchial epithelial cells to whole cigarette smoke mirror those of in-vivo exposed human bronchial mucosa.

ABSTRACT:

Background

Despite the well-known detrimental effects of cigarette smoke (CS), little is known about the complex gene expression dynamics in the early stages after exposure. This study aims to investigate early transcriptomic responses following CS exposure of airway epithelial cells in culture and compare these to those found in human CS exposure studies.

Methods

Primary bronchial epithelial cells (PBEC) were differentiated at the air-liquid interface (ALI) and exposed to whole CS. Bulk RNA-sequencing was performed at 1 h, 4 h, and 24 h hereafter, followed by differential gene expression analysis. Results were additionally compared to data retrieved from human CS studies.

Results

ALI-PBEC gene expression in response to CS was most significantly changed at 4 h after e

SUBMITTER: van der Does AM 

PROVIDER: S-EPMC9440516 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature