Induced hepatic stem cells maintain self-renewal through the high expression of Myc coregulated by TET1 and CTCF.

ABSTRACT:

Background

Induced hepatic stem cells (iHepSCs) with the capacities of self-renewal and bidifferentiation into hepatocytes and cholangiocytes were generated from mouse embryonic fibroblasts (MEFs) by lineage reprogramming in our previous research. However, the mechanism of iHepSC self-renewal has not been elucidated. Active demethylation regulated by Tet1 plays an important role in the self-renewal of stem cells, including pluripotent stem cells and adult stem cells. Here, we investigated the role and mechanism of Tet1-regulated demethylation in the self-renewal of iHepSCs.

Methods

The methylation levels and the expression of Tet1 in iHepSCs and MEFs were analyzed by immunofluorescent staining, quantitative reverse transcription PCR and western blotting. Then, the effects of

SUBMITTER: Wang C 

PROVIDER: S-EPMC9440563 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature