The allosteric gating mechanism of the MthK channel.

ABSTRACT: Allostery is a fundamental element during channel gating in response to an appropriate stimulus by which events occurring at one site are transmitted to distal sites to regulate activity. To address how binding of the first Ca²⁺ ion at one of the eight chemically identical subunits facilitates the other Ca²⁺-binding events in MthK, a Ca²⁺-gated K⁺ channel containing a conserved ligand-binding RCK domain, we analysed a large collection of MthK structures and performed the corresponding thermodynamic and electrophysiological measurements. These structural and functional studies led us to conclude that the conformations of the Ca²⁺-binding sites alternate between two quaternary states and exhibit significant differences in Ca²⁺

SUBMITTER: Guan F 

PROVIDER: S-EPMC9440719 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature