Inflammatory macrophages interrupt osteocyte maturation and mineralization via regulating the Notch signaling pathway.

ABSTRACT:

Background

It is well-known that both macrophages and osteocytes are critical regulators of osteogenesis and osteoclastogenesis, yet there is limited understanding of the macrophage-osteocyte interaction, and how their crosstalk could affect bone homeostasis and mineralization. This research therefore aims to investigate the effects of macrophage polarization on osteocyte maturation and mineralization process.

Methods

A macrophage-derived conditioned medium based osteocyte culture was set up to investigate the impact of macrophages on osteocyte maturation and terminal mineralization. Surgically induced osteoarthritis (OA) rat model was used to further investigate the macrophage-osteocyte interaction in inflammatory bone remodeling, as well as the involvement of the Notch sig

SUBMITTER: Wang S 

PROVIDER: S-EPMC9441044 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature