Calcitonin receptor antibody validation and expression in the rodent brain.

ABSTRACT:

Background and aim

Therapeutics that reduce calcitonin gene-related peptide activity are effective migraine treatments. However, gaps remain in our understanding of the molecular mechanisms that link calcitonin gene-related peptide to migraine. The amylin 1 receptor responds potently to calcitonin gene-related peptide, and to the related peptide amylin, but its role in relation to either peptide or to migraine is unclear. We sought to better understand the expression of the amylin 1 receptor protein subunit, the calcitonin receptor, in the rodent brain.

Methods

We profiled three antibodies for immunodetection of calcitonin receptor, using immunocytochemistry, western blotting, and calcitonin receptor conditional knockout mouse tissue. Selected migraine-relevant rat brain reg

SUBMITTER: Hendrikse ER 

PROVIDER: S-EPMC9441190 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature