Combined Transcriptomic and Metabolomic Analysis of Women with Polycystic Ovary Syndrome.

ABSTRACT:

Background

Polycystic ovary syndrome (PCOS) is a complex class of endocrine disorders with insulin resistance, compensatory hyperinsulinemia, and obesity. However, the pathogenesis and therapies of PCOS have not been fully elucidated. Exosomal miRNAs have the potential to serve as biomarkers and therapies for a wide range of medical conditions.

Method

We collected follicular fluid from 5 PCOS patients and 5 healthy people. High-throughput sequencing technology to identify differentially expressed miRNAs and untargeted metabolome identify differential metabolites in follicular fluid exosomal. RT-qPCR and AUC analysis were performed.

Result

miRNA high-throughput sequencing identified 124 differential miRNAs. RT-qPCR analysis confirmed the sequencing results. These diffe

SUBMITTER: Tian-Min Y 

PROVIDER: S-EPMC9441417 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature