Budding pouches and associated bubbles: 3D visualization of exo-membrane structures in plasmodium falciparum gametocytes.

ABSTRACT: Plasmodium falciparum gametocytes have unique morphology, metabolism, and protein expression profiles in their asexual stages of development. In addition to the striking changes in their appearance, a wide variety of "exo-membrane structures" are newly formed in the gametocyte stage. Little is known about their function, localization, or three-dimensional structural information, and only some structural data, typically two-dimensional, have been reported using conventional electron microscopy or fluorescence microscopy. For better visualization of intracellular organelle and exo-membrane structures, we previously established an unroofing technique to directly observe Maurer's clefts (MCs) in asexual parasitized erythrocytes by removing the top part of the cell's membrane followed by

SUBMITTER: Hayakawa ESH 

PROVIDER: S-EPMC9441640 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature