Microencapsulation and in situ incubation methodology for the cultivation of marine bacteria.

ABSTRACT: Environmental microorganisms are important sources of biotechnology innovations; however, the discovery process is hampered by the inability to culture the overwhelming majority of microbes. To drive the discovery of new biotechnology products from previously unculturable microbes, several methods such as modification of media composition, incubation conditions, single-cell isolation, and in situ incubation, have been employed to improve microbial recovery from environmental samples. To improve microbial recovery, we examined the effect of microencapsulation followed by in situ incubation on the abundance, viability, and diversity of bacteria recovered from marine sediment. Bacteria from marine sediment samples were resuspended or encapsulated in agarose and half of each samp

SUBMITTER: Pope E 

PROVIDER: S-EPMC9441948 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature