Transcriptomics Analysis Uncovers Transient Ceftazidime Tolerance in <i>Burkholderia</i> Biofilms.

ABSTRACT: Burkholderia pseudomallei is an etiological agent of melioidosis, a severe community-acquired infectious disease. B. pseudomallei strain K96243 is sensitive to the drug ceftazidime (CAZ), but has been shown to exhibit transient CAZ tolerance when in a biofilm form. To investigate an observed shift in gene expression profile during CAZ tolerance condition and to better understand the mechanistic aspects of this transient tolerance, RNA-sequencing was performed on B. pseudomallei K96243 from the following three states: planktonic, biofilm, and planktonic shedding. Results indicated that the expression of 651 genes (10.97%) were significantly changed in both biofilm (resistant) and planktonic shedding (sensitive) cells in comparison to the planktonic state. The top four h

SUBMITTER: Chattagul S 

PROVIDER: S-EPMC9443238 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature