DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection.

ABSTRACT: Zika virus (ZIKV) is a mosquito-borne virus that is phylogenetically close to other medically important flaviviruses with high global public health significance, such as dengue (DENV) and yellow fever (YFV) viruses. Correct diagnosis of a flavivirus infection can be challenging, particularly in world regions where more than one flavivirus co-circulates and YFV vaccination is mandatory. Acid nucleic aptamers are oligonucleotides that bind to a specific target molecule with high affinity and specificity. Because of their unique characteristics, aptamers are promising tools for biosensor development. Aptamers are usually obtained through a procedure called "systematic evolution of ligands by exponential enrichment" (SELEX). In this study, we select an aptamer (termed ZIKV60) by capillary elec

SUBMITTER: Almeida NBF 

PROVIDER: S-EPMC9443353 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature