Model-based intensification of CHO cell cultures: One-step strategy from fed-batch to perfusion.

ABSTRACT: There is a growing interest in continuous processing of the biopharmaceutical industry. However, the technology transfer from traditional batch-based processes is considered a challenge as protocol and tools still remain to be established for their usage at the manufacturing scale. Here, we present a model-based approach to design optimized perfusion cultures of Chinese Hamster Ovary cells using only the knowledge captured during small-scale fed-batch experiments. The novelty of the proposed model lies in the simplicity of its structure. Thanks to the introduction of a new catch-all variable representing a bulk of by-products secreted by the cells during their cultivation, the model was able to successfully predict cellular behavior under different operating modes without changes in its fo

SUBMITTER: Richelle A 

PROVIDER: S-EPMC9443430 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature