There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments.

ABSTRACT: Fluoride has been used in the internal recording solution for manual and automated patch clamp experiments for decades because it helps to improve the seal resistance and promotes longer lasting recordings. In manual patch clamp, fluoride has been used to record voltage-gated Na (Na_V) channels where seal resistance and access resistance are critical for good voltage control. In automated patch clamp, suction is applied from underneath the patch clamp chip to attract a cell to the hole and obtain a good seal. Since the patch clamp aperture cannot be moved to improve the seal like the patch clamp pipette in manual patch clamp, automated patch clamp manufacturers use internal fluoride to improve the success rate for obtaining GΩ seals. However, internal fluoride can affect voltage-

SUBMITTER: Rapedius M 

PROVIDER: S-EPMC9443850 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature