Evaluation of chemical compounds that inhibit neurite outgrowth using GFP-labeled iPSC-derived human neurons.

ABSTRACT: Due to the increasing number of drugs and untested environmental compounds introduced into commercial use, there is recognition for a need to develop reliable and efficient screening methods to identify compounds that may adversely impact the nervous system. One process that has been implicated in neurodevelopment is neurite outgrowth; the disruption of which can result in adverse outcomes that persist later in life. Here, we developed a green fluorescent protein (GFP) labeled neurite outgrowth assay in a high-content, high-throughput format using induced pluripotent stem cell (iPSC) derived human spinal motor neurons and cortical glutamatergic neurons. The assay was optimized for use in a 1536-well plate format. Then, we used this assay to screen a set of 84 unique compounds that have pre

SUBMITTER: Li S 

PROVIDER: S-EPMC9444042 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature