A rapid and standardized workflow for functional assessment of bacterial biosensors in fecal samples.

ABSTRACT: Gut metabolites are pivotal mediators of host-microbiome interactions and provide an important window on human physiology and disease. However, current methods to monitor gut metabolites rely on heavy and expensive technologies such as liquid chromatography-mass spectrometry (LC-MS). In that context, robust, fast, field-deployable, and cost-effective strategies for monitoring fecal metabolites would support large-scale functional studies and routine monitoring of metabolites biomarkers associated with pathological conditions. Living cells are an attractive option to engineer biosensors due to their ability to detect and process many environmental signals and their self-replicating nature. Here we optimized a workflow for feces processing that supports metabolite detection using bacterial b

SUBMITTER: Zuniga A 

PROVIDER: S-EPMC9444133 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature