Mapping ribonucleotides embedded in genomic DNA to single-nucleotide resolution using Ribose-Map.

ABSTRACT: The most common nonstandard nucleotides found in genomic DNA are ribonucleotides. Although ribonucleotides are frequently incorporated into DNA by replicative DNA polymerases, very little is known about the distribution and signatures of ribonucleotides incorporated into DNA. Recent advances in high-throughput ribonucleotide sequencing can capture the exact locations of ribonucleotides in genomic DNA. Ribose-Map is a user-friendly, standardized bioinformatics toolkit for the comprehensive analysis of ribonucleotide sequencing experiments. It allows researchers to map the locations of ribonucleotides in DNA to single-nucleotide resolution and identify biological signatures of ribonucleotide incorporation. In addition, it can be applied to data generated using any currently available high-th

SUBMITTER: Gombolay AL 

PROVIDER: S-EPMC9444191 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature