Identification of Somatic Proteins in Haemonchus Contortus Infective Larvae (L₃) and Adults.

ABSTRACT: Haemonchus contortus is considered the most pathogenic nematode in sheep production systems based on grazing. Comparing infective larvae (L₃) with adult parasites can lead to the identification of proteins that play an important role in parasite-host interactions. In this study, we report a list of H. contortus somatic proteins and made a comparative analysis of somatic proteins of L₃ and adult worms. L₃ and adult parasites were subjected to protein extraction and subsequently to peptide fractionation. Peptides were analysed by mass spectrometry and LC-MS/MS data analysis. Data analysis and search on SEQUEST and MASCOT against H. contortus from the WormBase ParaSite database resulted in the identification of 775 unique peptide sequences

SUBMITTER: Zaragoza-Vera M 

PROVIDER: S-EPMC9444209 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature