Determination of Five Coumarins in Angelicae Pubescentis Radix from Different Origins by HPTLC-Scanning.

ABSTRACT: The HPTLC method is widely used in the field of quality evaluation and component analysis of traditional Chinese medicine (TCM). This work developed an HPTLC method to determine the five effective components of osthole, columbianadin, isoimperatorin, oxypeucedanin, and imperatorin in Angelicae Pubescentis Radix (APR) from twelve different origins, and the quality difference was analyzed by comprehensive factor analysis and cluster analysis. The results showed that the calibration curves of five components exhibited good linearity within the linear ranges (0.8-4.0 μg). The RSD of precision was 1.06%-1.21%, and the repeatability and stability tests were good. The results of cluster analysis showed that the APR from 12 different areas was divided into two categories, and at the

SUBMITTER: Ji D 

PROVIDER: S-EPMC9444472 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature