Validation of a SARS-CoV-2 RT-PCR assay: a requirement to evaluate viral contamination in human semen.

ABSTRACT:

Research question

Is it possible to validate an accurate and reliable method for direct detection of SARS-CoV-2 by reverse transcription polymerase chain reaction (RT-PCR) in human semen fractions?

Design

This qualitative improvement study aimed to provide a prospective validation of SARS-CoV-2 detection in male semen. The SARS-CoV-2 genome was detected by multiplex real-time RT-PCR on patient samples that underwent routine semen analyses for infertility at the Center for Reproductive Medicine at the University Hospital of Clermont-Ferrand. Samples comprised surplus semen collected for treatment with assisted reproductive technology. Seminal fluid and spermatozoa fractions were isolated with density gradient centrifugation and cryopreserved. Positive samples were prepared wi

SUBMITTER: Chabrolles H 

PROVIDER: S-EPMC9444588 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature