Determination of FRET orientation factor between artificial fluorophore and photosynthetic light-harvesting 2 complex (LH2).

ABSTRACT: The orientation factor of fluorescence resonance energy transfer (FRET) between photosynthetic light-harvesting 2 complex (LH2) and artificial fluorophore (Alexa Fluor 647: A647) was theoretically investigated. The orientation factor of 2/3, i.e., the isotropic mean, is widely used to predict the donor-acceptor distance from FRET measurements. However, this approximation seems inappropriate because the movement of A647 is possibly restricted by the bifunctional linker binding to LH2. In this study, we performed molecular dynamics (MD) simulations and electronic coupling calculations on the LH2-A647 conjugate to analyze its orientation factor. The MD results showed that A647 keeps a position approximately 26 Å away from the bacteriochlorophyll (BChl) assembly in LH2. The effective orientati

SUBMITTER: Fujimoto KJ 

PROVIDER: S-EPMC9445053 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature