Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids.

ABSTRACT: The development dynamics and self-organization of glandular branched epithelia is of utmost importance for our understanding of diverse processes ranging from normal tissue growth to the growth of cancerous tissues. Using single primary murine pancreatic ductal adenocarcinoma (PDAC) cells embedded in a collagen matrix and adapted media supplementation, we generate organoids that self-organize into highly branched structures displaying a seamless lumen connecting terminal end buds, replicating in vivo PDAC architecture. We identify distinct morphogenesis phases, each characterized by a unique pattern of cell invasion, matrix deformation, protein expression, and respective molecular dependencies. We propose a minimal theoretical model of a branching and proliferating tissue, capturing the dy

SUBMITTER: Randriamanantsoa S 

PROVIDER: S-EPMC9445099 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature