Leptolysin, a Leptospira secreted metalloprotease of the pappalysin family with broad-spectrum activity.

ABSTRACT: Extracellular proteolytic enzymes are produced by a variety of pathogenic microorganisms, and contribute to host colonization by modulating virulence. Here, we present a first characterization of leptolysin, a Leptospira metalloprotease of the pappalysin family identified in a previous exoproteomic study. Comparative molecular analysis of leptolysin with two other pappalysins from prokaryotes, ulilysin and mirolysin, reveals similarities regarding calcium, zinc, and arginine -binding sites conservation within the catalytic domain, but also discloses peculiarities. Variations observed in the primary and tertiary structures may reflect differences in primary specificities. Purified recombinant leptolysin of L. interrogans was obtained as a ~50 kDa protein. The protease exhibite

SUBMITTER: Courrol DDS 

PROVIDER: S-EPMC9445424 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature