High-throughput, real-time monitoring of engineered skeletal muscle function using magnetic sensing.

ABSTRACT: Engineered muscle tissues represent powerful tools for examining tissue level contractile properties of skeletal muscle. However, limitations in the throughput associated with standard analysis methods limit their utility for longitudinal study, high throughput drug screens, and disease modeling. Here we present a method for integrating 3D engineered skeletal muscles with a magnetic sensing system to facilitate non-invasive, longitudinal analysis of developing contraction kinetics. Using this platform, we show that engineered skeletal muscle tissues derived from both induced pluripotent stem cell and primary sources undergo improvements in contractile output over time in culture. We demonstrate how magnetic sensing of contractility can be employed for simultaneous assessment of multiple ti

SUBMITTER: Smith AS 

PROVIDER: S-EPMC9445471 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature