Mini Bioreactor Can Support In Vitro Spermatogenesis of Mouse Testicular Tissue.

ABSTRACT:

Objective

It was in the early 20^th century when the quest for in vitro spermatogenesis started. In vitro spermatogenesis is critical for male cancer patients undergoing gonadotoxic treatment. Dynamic culture system creates in vivo-like conditions. In this study, it was intended to evaluate the progression of spermatogenesis after testicular tissue culture in mini-perfusion bioreactor.

Materials and methods

In this experimental study, 12 six-day postpartum neonatal mouse testes were removed and fragmented, placed on an agarose gel in parallel to bioreactor culture, and incubated for 8 weeks. Histological, molecular and immunohistochemical evaluations were carried out after 8 weeks.

Results

Histological analysis suggested successful maintenance

SUBMITTER: Amirkhani Z 

PROVIDER: S-EPMC9445517 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature