Competitive fitness of Pseudomonas aeruginosa isolates in human and murine precision-cut lung slices.

ABSTRACT: Chronic respiratory infections with the gram-negative bacterium Pseudomonas aeruginosa are an important co-morbidity for the quality of life and prognosis of people with cystic fibrosis (CF). Such long-term colonization, sometimes lasting up to several decades, represents a unique opportunity to investigate pathogen adaptation processes to the host. Our studies aimed to resolve if and to what extent the bacterial adaptation to the CF airways influences the fitness of the pathogen to grow and to persist in the lungs. Marker-free competitive fitness experiments of serial P. aeruginosa isolates differentiated by strain-specific SNPs, were performed with murine and human precision cut lung slices (PCLS). Serial P. aeruginosa isolates were selected from six mild and six sev

SUBMITTER: Cramer N 

PROVIDER: S-EPMC9446154 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature