Self-assembly of globular proteins with intrinsically disordered protein polyelectrolytes and block copolymers.

ABSTRACT: Intrinsically disordered polypeptides are a versatile class of materials, combining the biocompatibility of peptides with the disordered structure and diverse phase behaviors of synthetic polymers. Synthetic polyelectrolytes are capable of complex phase behavior when mixed with oppositely charged polyelectrolytes, facilitating nanoparticle formation and bulk phase separation. However, there has been limited exploration of intrinsically disordered protein polyelectrolytes as potential bio-based replacements for synthetic polyelectrolytes. Here, we produce negatively charged, intrinsically disordered polypeptides, capable of high-yield expression in E. coli and use this intrinsically disordered peptide to produce entirely protein-based polyelectrolyte complexes. The complexes display

SUBMITTER: Horn JM 

PROVIDER: S-EPMC9446422 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature