Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood.

ABSTRACT:

Background

Sepsis is caused mainly by infection in the blood with a broad range of bacterial species. It can be diagnosed by molecular diagnostics once compounds in the blood that interfere with molecular diagnostics are removed. However, this removal relies on ultracentrifugation. Immunomagnetic separation (IMS), which typically uses antibody-conjugated silica-coated magnetic nanoparticles (Ab-SiO₂-MNPs), has been widely applied to isolate specific pathogens in various types of samples, such as food and environmental samples. However, its direct use in blood samples containing bacteria is limited due to the aggregation of SiO₂-MNPs in the blood and inability to isolate multiple species of bacteria causing sepsis.

Results

In this study, we report the sy

SUBMITTER: Abafogi AT 

PROVIDER: S-EPMC9446563 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature