Determining the optimal stage for cryopreservation of human embryonic stem cell-derived retinal pigment epithelial cells.

ABSTRACT:

Background

Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-derived RPE) are a promising source for cell-replacement therapy to treat retinal degenerative diseases, but research on RPE cryopreservation is limited. This study aimed to determine the best phase for RPE cryopreservation to preserve the post-thaw function and uncover the mechanism underlying RPE freezing tolerance.

Methods

hESC-derived RPE cells were cryopreserved at various time points after seeding. After thawing, the survival and attachment rates, RPE marker gene expression, apical-basal polarity, PEDF secretion, transepithelial resistance, and phagocytotic ability of post-thaw RPE cells were evaluated. RNA sequencing was performed on RPE cells at three-time points, differentially expre

SUBMITTER: Zhang T 

PROVIDER: S-EPMC9446586 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature