Tau seeding activity in various regions of down syndrome brain assessed by two novel assays.

ABSTRACT: Propagation of tau pathology via the seeding of naive tau aggregation underlies the progression of Alzheimer's disease (AD) and related tauopathies. Individuals with Down syndrome (DS) develop tau pathology at the fourth decade of life, but tau seeding activity in DS brain has not yet been determined. To measure tau seeding activity, we developed capture assay and seeded-tau aggregation assay with truncated tau_151-391. By using brain extracts from AD and related tauopathies, we validated these two methods and found that the brain extracts from AD and related tauopathies, but not from controls and the diseases in which tau was not hyperphosphorylated, captured in vitro and seeded 3R-tau_151-391 and 4R-tau_151-391 to aggregate in cultured cells similarly. Captu

SUBMITTER: Jin N 

PROVIDER: S-EPMC9446852 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature