High-yield bioactive triterpenoid production by heterologous expression in Nicotiana benthamiana using the Tsukuba system.

ABSTRACT: Oleanolic acid is a pentacyclic triterpenoid found in numerous plant species and is a precursor to several bioactive triterpenoids with commercial potential. However, oleanolic acid accumulates at low levels in plants, and its chemical synthesis is challenging. Here, we established a method for producing oleanolic acid in substantial quantities via heterologous expression of pathway enzymes in Nicotiana benthamiana. The "Tsukuba system" is one of the most efficient agroinfiltration-based transient protein expression systems using the vector pBYR2HS, which contains geminiviral replication machinery and a double terminator for boosting expression. Additionally, the pBYR2HS vector contains an expression cassette for the gene-silencing suppressor p19 protein from tomato bushy stu

SUBMITTER: Romsuk J 

PROVIDER: S-EPMC9447470 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature