MiR-31 improves spinal cord injury in mice by promoting the migration of bone marrow mesenchymal stem cells.

ABSTRACT:

Background

Stem cell transplantation therapy is a potential approach for the repair of spinal cord injuries and other neurodegenerative diseases, but its effectiveness is hampered by the low rate of targeted migration of cells to the area of injury. The aim of this study was to investigate the effects of miR-31 on the migration of bone marrow mesenchymal stem cells (BMSCs) and the regulation of MMP-2 and CXCR4 expression in vitro and in vivo.

Methods

eGFP-expressing BMSCs were isolated and cultured for subsequent experiments. The experiments were divided into three groups: control group, miR-31agomir group, and miR-31antagomir group. Proliferation was analyzed using CCK-8 and flow cytometry; cell migration in vitro was analyzed using wound-healing and transwell assays. The m

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9447891 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature