Methylation levels assessment with Methylation-Sensitive High-Resolution Melting (MS-HRM).

ABSTRACT: Testing for disease-related DNA methylation changes provides clinically relevant information in personalized patient care. Methylation-Sensitive High-Resolution Melting (MS-HRM) is a method used for measuring methylation changes and has already been used in diagnostic settings. This method utilizes one set of primers that initiate the amplification of both methylated and non-methylated templates. Therefore, the quantification of the methylation levels using MS-HRM is hampered by the PCR bias phenomenon. Some approaches have been proposed to calculate the methylation level of samples using the high-resolution melting (HRM) curves. However, limitations of the methylation calculation using MS-HRM have not been evaluated systematically and comprehensively. We used the Area Under the Curve (AUC

SUBMITTER: Samso Mathiasen S 

PROVIDER: S-EPMC9447921 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature