Identification of an effector specificity subregion within the aromatic-responsive regulators DmpR and XylR by DNA shuffling.

ABSTRACT: The Pseudomonas derived sigma(54)-dependent regulators DmpR and XylR control the expression of genes involved in catabolism of aromatic compounds. Binding to distinct, nonoverlapping groups of aromatic effectors controls the activities of these transcriptional activators. Previous work has derived a common mechanistic model for these two regulators in which effector binding by the N-terminal 210 residues (the A-domain) of the protein relieves repression of an intrinsic ATPase activity essential for its transcription-promoting property and allows productive interaction with the transcriptional apparatus. Here we dissect the A-domains of DmpR and XylR by DNA shuffling to identify the region(s) that mediates the differences in the effector specificity profiles. Analysis of in vivo transcripti

SUBMITTER: Skarfstad E 

PROVIDER: S-EPMC94483 | biostudies-literature | 2000 Jun

REPOSITORIES: biostudies-literature