Synergistic Lethality Effects of Apatinib and Homoharringtonine in Acute Myeloid Leukemia.

ABSTRACT:

Purpose

The significance of vascular endothelial growth factor receptor (VEGFR)-2 in numerous solid tumors and acute myeloid leukemia (AML) has been demonstrated, but Apatinib remains largely unexplored. In this study, whether Apatinib combined with homoharringtonine (HHT) kills AML cell lines and its possible mechanisms have been explored.

Methods

AML cell lines were treated with Apatinib and HHT in different concentrations with control, Apatinib alone, HHT alone, and Apatinib combined with HHT. The changes of IC₅₀ were measured by CCK8 assay, and apoptosis rate, cell cycle, and the mitochondrial membrane potential in each group were measured by flow cytometry. Finally, the possible cytotoxicity mechanism was analyzed by Western blotting.

Results

Our resul

SUBMITTER: Shi Y 

PROVIDER: S-EPMC9448536 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature