Qiliqiangxin Capsule Modulates Calcium Transients and Calcium Sparks in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

ABSTRACT:

Background

The therapeutic effects of Qiliqiangxin capsule (QLQX), a Chinese patent medicine, in patients with chronic heart failure are well established. However, whether QLQX modulates cardiac calcium (Ca²⁺) signals, which are crucial for the heart function, remains unclear. Aim of the Study. This study aimed to evaluate the role of QLQX in modulating Ca²⁺ signals in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).

Materials and methods

Fluorescence imaging was used to monitor Ca²⁺ signals in the cytosol and nuclei of hiPSC-CMs. For Ca²⁺ spark measurements, the line-scan mode of a confocal microscope was used.

Results

The QLQX treatment substantially decreased the frequency of spontaneous Ca

SUBMITTER: Li Y 

PROVIDER: S-EPMC9448542 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature