Generation of an Escherichia coli strain growing on methanol via the ribulose monophosphate cycle.

ABSTRACT: Methanol is a liquid with high energy storage capacity that holds promise as an alternative substrate to replace sugars in the biotechnology industry. It can be produced from CO₂ or methane and its use does not compete with food and animal feed production. However, there are currently only limited biotechnological options for the valorization of methanol, which hinders its widespread adoption. Here, we report the conversion of the industrial platform organism Escherichia coli into a synthetic methylotroph that assimilates methanol via the energy efficient ribulose monophosphate cycle. Methylotrophy is achieved after evolution of a methanol-dependent E. coli strain over 250 generations in continuous chemostat culture. We demonstrate growth on methanol and biomass formation exclus

SUBMITTER: Keller P 

PROVIDER: S-EPMC9448777 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature