MicroRNA analysis reveals the role of miR-214 in duck adipocyte differentiation.

ABSTRACT:

Objective

Fat deposition in poultry is an important factor in production performance and meat quality research. miRNAs also play important roles in regulating adipocyte differentiation process. This study was to investigate the expression patterns of miRNAs in duck adipocytes after differentiation and explore the role of miR-214 in regulating carnitine palmitoyltransferases 2 (CPT2) gene expression during duck adipocyte differentiation.

Methods

Successful systems for the isolation, culture, and induction of duck primary fat cells was developed in the experiment. Using Illumina next-generation sequencing, the miRNAs libraries of duck adipocytes were established. miRanda was used to predict differentially expressed (DE) miRNAs and their target genes. The expression patterns of

SUBMITTER: Wang L 

PROVIDER: S-EPMC9449393 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature