Specific microRNAs are associated with fracture healing phases, patient age and multi-trauma.

ABSTRACT:

Background

Immediately after a fracture occurs, a fracture hematoma (fxH) is formed. This fxH plays an important role in fracture healing and, under normal circumstances, aids in generating an environment in which a wide variety of cells orchestrate processes involved in fracture healing. MicroRNAs (miRNAs) may influence these processes. The aim of this study was therefore to determine the miRNA expression signature of human fxH in normal fracture healing and examine the potential influence of clinical parameters on these expression levels.

Methods

fxH was harvested from 61 patients (mean age 52 ± 19; 32♀) during fracture surgery. miRNAs were isolated, transcribed and pooled for qPCR array analysis and validation. Qiagen fibrosis- and inflammation qPCR arrays w

SUBMITTER: Groven RVM 

PROVIDER: S-EPMC9449672 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature