Proteome Phenotypes Discriminate the Growing Location and Malting Traits in Field-Grown Barley.

ABSTRACT: Barley is one of the key cereal grains for malting and brewing industries. However, climate variability and unprecedented weather events can impact barley yield and end-product quality. The genetic background and environmental conditions are key factors in defining the barley proteome content and malting characteristics. Here, we measure the barley proteome and malting characteristics of three barley lines grown in Western Australia, differing in genetic background and growing location, by applying liquid chromatography-mass spectrometry (LC-MS). Using data-dependent acquisition LC-MS, 1571 proteins were detected with high confidence. Quantitative data acquired using sequential window acquisition of all theoretical (SWATH) MS on barley samples resulted in quantitation of 920 proteins. Mult

SUBMITTER: Bahmani M 

PROVIDER: S-EPMC9449971 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature