Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis.

ABSTRACT:

Background

Acute pancreatitis is the sudden inflammation of the pancreas. Severe cases of acute pancreatitis are potentially fatal and have no specific treatment available. Premature trypsinogen activation could initiate acute pancreatitis. However, the mechanism underlying premature trypsinogen activation is not fully understood.

Methods

In this research, a primary pancreatic acinar cell or mouse acute pancreatitis model was constructed. The effect of acid ceramidase (ASAH1), which is responsible for sphingosine production, was investigated in trypsinogen activation in vitro and in vivo. Meanwhile, the proteins regulating ASAH1 or binding to sphingosine were also detected by co-immunoprecipitation followed by mass spectrometry.

Results

The results showed that ASAH1 i

SUBMITTER: Xiao J 

PROVIDER: S-EPMC9450262 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature