Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells.

ABSTRACT:

Background

Citrate Synthase (Cs) gene mutation (locus ahL4) has been found to play an important role in progressive hearing loss of A/J mice. HEI-OC1 cells have been widely used as an in vitro system to study cellular and molecular mechanisms related to hearing lose. We previously reported the increased apoptosis and the accumulation of reactive oxygen species in shRNACs-1429 cells, a Cs low-expressed cell model from HEI-OCI. The details of the mechanism of ROS production and apoptosis mediated by the abnormal expression of Cs needed to research furtherly.

Methods

iTRAQ proteomics was utilized to detect the differentially expressed proteins (DEPs) caused by low expression of Cs. The GO and KEGG pathways analysis were performed for annotation of the differentially expressed p

SUBMITTER: Xu X 

PROVIDER: S-EPMC9450364 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature